Biological Applications and Limitations of Electron Microscope

Limitations of electron microscope:

Even though electron microscope is a great advancement over the light microscopes in terms of higher magnification and better resolution, there are certain limitations and light microscopes have their own useful role to play in the microbiological laboratory. The limitations imposed by electron microscope are:-

1. Hydrated specimens are not to be used; hence live specimens cannot be observed

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2. Dehydration even if carefully done will cause some distortion

3. Artifacts may appear

4. As electrons have a low penetration power extremely thin sections are necessary.